Today I was supposed to do a whole experiment from start to almost finish with PCR. I was extremely tired today, so I stopped at Starbucks on my way to work to get an iced coffee (I have a giftcard that needs to be used). I ended up doing ligation wrong, because we were using a new program on the Thermocycler called Gibsen, and I accidentally used the ligation program. But all is well, my mentor helped me fix it. Then, I did transformation wrong. You have to wait an hour and incubate the heatshocked cells, but you mix the cells with growth media. However, I incubated but forgot to add broth. So I waited the whole hour (which was from 7:30pm to 8:30pm), only to find that I did it wrong, I had to stay in the lab for so late, and I would have to do it again. I plated the solution just to see if any cells would grow overnight.
So today was just a rough day, but tomorrow will be better with happy hour (to be explained in tomorrow's post).
Edit: no cells grew.
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