So today transformation didn't work again. One colony again. However, we found out why it wasn't working. I was using the wrong bacterial cells. I have been for this whole time, which explains why all the transformations I've done haven't worked out. I've been using cells that aren't that good for transformation. Well, that makes me feel better now.
Also today, my mentor and I were good labmates to the rest of our lab, and so we defrosted the freezer. Which sounds cooler than it really is. We basically took the tame version of icepicks and hacked off frost and snow (yes, snow in summer!) off the whole freezer inside. I was sprayed with flying snow and ice. Brrr. Plus, the snow/ice was so cold, it burned my hands through my latex gloves. Oops, they were red afterwards. Taking one for the lab though, so I did good.
My practice cell line was discarded by my mentor but I got upgraded to 4 that is part of my project. So much pressure not to mess it all up now...two weeks (minus a day) until poster day!
After work today. Olivia (girl from the elevator) and I went to play tennis. It's nice to play with someone my age (senior this fall) and despite being very different personality wise, we still have a lot to talk about and I got more tennis practice in!
Week 7 is finished and 2 more weeks remain. It's been a crazy summer but the ultimate craziness is yet to come, so stay tuned! Everything will be driving towards the ultimate day at NIH and there's so many more things I need to see in DC before I head home.
No comments:
Post a Comment